17 September 2021

Gutekunst and colleagues, 2021

Communications Biology
Gutekunst J, Maiakovska O, Hanna K, Provataris P, Horn H, Wolf S, Skelton CE, Dorn NJ, Lyko F. 2021. Phylogeographic reconstruction of the marbled crayfish origin. Communications Biology 4(1): 1096. https://doi.org/10.1038/s42003-021-02609-w

 

Abstract

 

The marbled crayfish (Procambarus virginalis) is a triploid and parthenogenetic freshwater crayfish species that has colonized diverse habitats around the world. Previous studies suggested that the clonal marbled crayfish population descended as recently as 25 years ago from a single specimen of P. fallax, the sexually reproducing parent species. However, the genetic, phylogeographic, and mechanistic origins of the species have remained enigmatic. We have now constructed a new genome assembly for P. virginalis to support a detailed phylogeographic analysis of the diploid parent species, Procambarus fallax. Our results strongly suggest that both parental haplotypes of P. virginalis were inherited from the Everglades subpopulation of P. fallax. Comprehensive whole-genome sequencing also detected triploid specimens in the same subpopulation, which either represent evolutionarily important intermediate genotypes or independent parthenogenetic lineages arising among the sexual parent population. Our findings thus clarify the geographic origin of the marbled crayfish and identify potential mechanisms of parthenogenetic speciation.

 

Keywords: None provided.

29 August 2021

Tönges and colleagues 2021

Frontiers in Ecology and Evolution
Tönges S, Masagounder K, Lenich F, Gutekunst J, Tönges M, Lohbeck J, Miller AK, Böhl F, Lyko F. 2021. Evaluating invasive marbled crayfish as a potential livestock for sustainable aquaculture. Frontiers in Ecology and Evolution 9: 651981. https://www.frontiersin.org/article/10.3389/fevo.2021.651981


Abstract

 

The marbled crayfish (Procambarus virginalis) is a recently discovered freshwater crayfish species, which reproduces by apomictic parthenogenesis, resulting in a monoclonal, and all-female population. The animals were widely distributed through the aquarium trade and have established numerous stable wild populations through anthropogenic releases. They are highly prevalent in Madagascar, where they have become a popular source of nutritional protein. As freshwater crayfish aquaculture in open systems is a thriving, but ecologically damaging global industry, alternatives are urgently needed. Although marbled crayfish are often branded by their invasive mode of reproduction, their overall invasiveness is not higher than for other cultured crayfish species. Furthermore, their resiliency and high adaptability provide a strong rationale for evaluating them for closed, and environmentally safe aquaculture approaches. Here we describe a novel population of marbled crayfish in a former German coal mining area that is characterized by acid and polluted water. Even under these adverse conditions, animals grew to sizes, and weights that are comparable to commercially farmed freshwater crayfish. Tailored feed development and laboratory testing demonstrated highly efficient feed conversion, suggesting a considerable capacity for sustainable production in closed systems. We further show that marbled crayfish meat can be readily introduced into European meals. Finally, chemical analysis of marbled crayfish exoskeletons revealed comparably high amounts of chitin, which is a valuable source for the synthesis of chitosan and bioplastics. Our results thus suggest that production of marbled crayfish in closed systems may represent a sustainable alternative for crayfish aquaculture.


Keywords: livestock • invasive species • feed conversion • chitin • sustainability • aquaculture • marbled crayfish • tailored feeds


Open access



Francesconi and colleagues 2021

Frontiers in Evology and Evolution.
Francesconi C, Makkonen J, Schrimpf A, Jussila J, Kokko H, Theissinger K. 2021. Controlled infection experiment with Aphanomyces astaci provides additional evidence for latent infections and resistance in freshwater crayfish. Frontiers in Ecology and Evolution 9: 647037. https://doi.org/10.3389/fevo.2021.647037

 

Abstract

 

For 150 years the crayfish plague disease agent Aphanomyces astaci has been the cause of mass mortalities among native European crayfish populations. However, recently several studies have highlighted the great variability of A. astaci virulence and crayfish resistance toward the disease. The main aim of this study was to compare the response of two crayfish species, the European native noble crayfish (Astacus astacus) and the invasive alien marbled crayfish (Procambarus virginalis), to an A. astaci challenge with a highly virulent strain from haplogroup B and a lowly virulent strain from haplogroup A. In a controlled infection experiment we showed a high resistance of marbled crayfish against an A. astaci infection, with zoospores from the highly virulent haplogroup B strain being able to infect the crayfish, but unable to cause signs of disease. Furthermore, we demonstrated a reduced virulence in the A. astaci strain belonging to haplogroup A, as shown by the light symptoms and the lack of mortality in the generally susceptible noble crayfish. Interestingly, in both marbled crayfish and noble crayfish challenged with this strain, we observed a significant decrease of the detected amount of pathogen’s DNA during the experiment, suggesting that this A. astaci haplogroup A strain has a decreased ability of penetrating into the cuticle of the crayfish. Our results provide additional evidence of how drastically strains belonging to A. astaci haplogroup B and haplogroup A differ in their virulence. This study confirmed the adaptation of one specific A. astaci haplogroup A strain to their novel European hosts, supposedly due to reduced virulence. This feature might be the consequence of A. astaci’s reduced ability to penetrate into the crayfish. Finally, we experimentally showed that marbled crayfish are remarkably resistant against the crayfish plague disease and could potentially be latently infected, acting as carriers of highly virulent A. astaci strains.

 

Keywords: marbled crayfish • noble crayfish • host-pathogen co-evolution • crayfish plague • experimental infection

OPen access

25 August 2021

Fishing for Marmorkrebs

Man in pond with nets and buckets fishing for crayfish.

I missed a story from back in spring about how Marmorkrebs are being commercially fished in Berlin.


German newspaper Die Zeit reports that Marmorkrebs are an addition to an existing crayfishing operation. About a ton of Lousiana red swamp crayfish have been caught every year over the last few years. 


NPR reported on this a couple of years ago, and did not paint a rosy picture of the fishery. Despite jokes about “How can they be a problem if we can eat them?” every time I talk about invasive crayfish, not everyone likes to eat them. Few restaurants are interested, and supply is irregular.


We’ll see.


External links


Erstmals sollen Marmorkrebse ins Netz gehen


For Berlin, invasive crustaceans are a tough catch and a tough sell

09 August 2021

Lemmer and colleagues, 2020

Lemmers P, Spikmans F, Volk L. 2020. De Marmerkreeft (Procambarus virginalis), een nieuwe invasieve exoot in Limburg. Natuurhistorisch Maandblad 109: 260-266.

 

Abstract

 

In terms of crayfish, the province of Limburg is a province poor in species by Dutch standards. Until 2020, only three invasive alien crayfish species were known. However, Marbled crayfish (Procambarus virginalis) were found at two isolated locations near Venlo within a short period of time in the spring of 2020. The origin and release of one of the populations could be traced via an internet vendor, who had released ten animals in 2017. A dipnet survey in 2020 at this site showed that the density was at least 30.8 specimens per 100 m of embankment. At the other site, length-frequency data suggest the species has been present since 2018. Here, the density was estimated at 5.4 individuals per 100 m of embankment. Further expansion of the populations via overland migration can be expected. It is likely that negative effects on nearby native amphibian populations will occur when no action is taken. The conclusions drawn from this study are that Marbled crayfish (1) are still offered for sale in the aquarium trade even after EU legislation, (2) are actively being released into the wild and (3) are able to establish populations in the Dutch countryside.

29 July 2021

Lemmer and colleagues, 2021

Cover to Living Nature, volume 122, issue 4.
Lemmers P, Frank Spikmans F, Koese B. 2021. Is de opmars van de marmerkreeft in Nederland nog te stuiten? De Levende Natuur 122(4): 138-140. https://delevendenatuur.nl/tijdschrift/2021-4

 

Abstract

 

The first marbled crayfish in the Netherlands was recorded in 2004. The next was observed a decade later, in 2014. Since then the number of observations has increased significantly up to 14 sites in 2020, spread all over the country. The marbled crayfish became established in at least four sites of which one has been eliminated in 2020. Animals are mainly found in vegetation- rich, shallow parts of isolated waters. Other crayfish species were not present in these sites. The main introduction route of marbled crayfish is release by pet keepers. The EU Regulation 1143/2014 on Invasive Alien Species seems to have limited effect on the ongoing invasion of this species yet, as both trade and new introductions are still taking place. Swift actions, considered both cost effective and mandatory under EU legislation, are urgently needed to eliminate the species while this is still feasible.

 

Note: The main text of article is in Dutch. The English title is roughly, “The expansion of the marbled crayfish in the Netherlands.”

24 July 2021

Illinois State University logo.
DeMaegd ML. 2021. Physiological consequences of neuromodulation and the cellular properties that underlie them. Dissertation for Doctor of Philosophy (PhD), School of Biological Sciences, Illinois State University. https://doi.org/10.30707/ETD2021.20210719070603173187.87

 

Abstract

 

Neuronal activity is a product of more than the underlying neuronal connections. Modulatory influences like changes in the animal’s environment, the animals physiological state, or the release of neuromodulators can dramatically alter neuronal activity. Modulatory influences can be beneficial for the animal because they are a source of neuronal and behavioral plasticity, and they can provide neuronal circuits with the robustness needed to continue to function in new conditions, states, or tasks.However, malfunctions of the modulatory system can disrupt neuronal activity and lead to pathologies. Predicting how modulatory influences will alter neuronal activity is challenging because the underlying cellular and circuit properties are delicately balanced and often respond in nonlinear and multifaceted ways to modulatory influences. In my thesis I address how several types of modulatory influences affect neuronal activity in the crustacean stomatogastric nervous system, and seek to characterize the circuit and cellular mechanisms that underlie them. In Chapter II I show that the activation of chemosensory pathway alters the frequency of backpropagating action potentials in a proprioceptive sensory neuron that measures muscle tension when the animal chews. These backpropagating action potentials invade the most distant regions of the proprioceptive neuron where muscle tension is encoded, including the spike initiation site and sensory dendrites. They alter the latency, the number, and the frequency of action potentials in response to muscle stimuli. When the chemosensory neurons become active, backpropagating action potential frequency decreases, thereby granting greater sensitivity to the muscle tension receptor. Since the chemosensory pathway is activated by food before the chewing starts, the modulation of backpropagating action potentials prepares the muscle receptor for future changes in muscle tension. Thus, my results demonstrate that one sensory pathway can prime another for upcoming tasks via the modulation of backpropagating action potentials. In Chapter III I show two ways that neuronal activity can be sustained during temperature modulation. First, I show that axons of different pyloric neurons maintain action potential timing between them over a large temperature range, despite their distinct morphological and intrinsic properties. I used computational model axons to determine if, and if so, how, axons with different diameters that are exposed to varying temperatures can maintain action potential timing with one another. I found that the temperature sensitivity of most ion channel properties mattered little to action potential timing. Conversely, the ratio of two Sodium channel parameters were critical: how much the maximum conductance and activation gate time constant in one axon changed with temperature relative to the other axon strongly influenced action potential timing between two. Since the ratio was critical, but not the actual values, this demonstrated that even highly temperature-sensitive ion channels can support temperature-robust action potential timing between neurons. Second, I show that acutely warming the stomatogastric ganglion by 3°C disrupts a gastric mill rhythm by diminishing the spread of electrical signals in the dendrites of the Lateral Gastric neuron (LG). I also show that a substance P-related peptide restores dendritic electrical spread and consequently the gastric mill rhythm at the warmer temperature. Specifically, the peptide rescues electrical spread through the activation of a modulatory cation current (the 'modulatory induced current' (IMI)). These data demonstrate the cellular mechanisms by which this peptide neuromodulator induces temperature-robust neuronal activity. A realization during my work on the previous chapters was that few peer-reviewed protocols exist that provide detailed and reproducible workflows of electrophysiological and molecular approaches for the study of modulatory influences. Many laboratories use 'homegrown' protocols or protocols that were inherited by word of mouth and are not widely available. This leads to a lack or reproducibility of research approaches and results and impedes the widespread use of these techniques. Chapters IV and V address these issues. In Chapter IV, I first, provide detailed protocols on how to generate action potentials in an axon using extracellular stimulation. Second, I provide a detailed protocol on how to measure action potential conduction velocity using extracellular recordings. In Chapter V, I expand on the concept of providing easily understandable and reproducible protocols to the processes of integrating genetic and molecular techniques with electrophysiological one in both lab and classroom settings. I establish a workflow that guides undergraduates or physiologists in the manual identification, confirmation, and curation of putative genes involved in neuronal function. I implement this workflow in a Course in Undergraduate Research Education (CURE) – like setting, that brings undergraduate students of all levels to actively participate in research labs by allowing students to work under supervision of graduate students and faculty mentors. The workflow outlines a efficient protocol for gene identification in marbled crayfish, clear leaning objectives, and several quality control and assessment processes that enable students to conceptualize the interconnectedness of genetics, molecular, and physiological neuroscience. By following this workflow, I identified the transcript and gene sequences for two Gamma Aminobutyric Acid (GABA) receptors subunits in the marbled crayfish (Procambarus virginalis). In addition to its educational purpose, the provided protocol serves as a first step toward integrating genetic and molecular techniques with electrophysiological ones to study the impact of receptor diversity for the cellular mechanisms of modulation in the marbled crayfish.

 

Keywords: None provided. 

 

Note: This document is embargoed until June 28, 2022.

25 June 2021

Sanna and colleagues, 2021

Logo for journal "Life".
Sanna D, Azzena I, Scarpa F, Cossu P, Pira A, Gagliardi F, Casu M. 2021. First record of the alien species Procambarus virginalis Lyko, 2017 in fresh waters of Sardinia and insight into its genetic variability. Life 11(7): 606. https://doi.org/10.3390/life11070606

 

Abstract

 

In the fresh waters of Sardinia (Italy), the non-indigenous crayfish species Procambarus clarkii has been reported from 2005, but, starting from 2019, there have been several reports of a new nonindigenous crayfish in southern and central areas of this Mediterranean island, and its morphology suggests that this species may be the marbled crayfish Procambarus virginalis. Forty-seven individuals of this putative species were analyzed, using the mitochondrial gene Cytochrome c Oxidase subunit I as molecular marker to identify this crayfish and investigate the level of genetic variability within the recently established population. Phylogenetic and phylogeographic analyses were carried out on a dataset including sequences from the Sardinian individuals and from all congenerics available in GenBank. Results showed that the new Sardinian crayfish belong to the species P. virginalis. All the sequences belonging to P. virginalis from European countries are identical, with only few exceptions found among Sardinian individuals. In conclusion, this paper highlights the occurrence of a new further alien species in the Sardinian fresh waters, which are already characterized by the high presence of non-indigenous species.

 

Keywords: alien species • invasive species • non-indigenous crayfish • biological invasion • marbled crayfish • Sardinian fresh waters • mtDNA

 

Open access

 

19 June 2021

Fun for people of all ages! Marmorkrebs colouring

I stumbled across a page with a coloring book style blank illustration of Marmorkrebs! While the page says it’s for kids, there’sa a lot of fine detail that makes me think some young ones may find it a little on the high end of the difficulty scale.

 

Black and white outline of crayfish for colouring.

 

The shape of the claws looks much more like Louisiana red swamp crayfish than Marmorkrebs, though.

01 June 2021

Muuga 2021

Muuga J-M. 2021. Effects of temperature on marbled crayfish (Procambarus virginalis, Lyko 2017) invasion ecology. Master’s thesis, Hydrobiology and Fishery, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences. http://hdl.handle.net/10492/6695

 

Abstract

 

Biological invasions are crucial issues worldwide and marbled crayfish (Procambarus virginalis, Lyko 2017) is one of the examples of freshwater invaders spreading across Europe and beyond. Marbled crayfish has high growth rate and reproduces via parthenogenesis, making it unique among other decapod species. Even though it is a warm water species, they can resist colder temperatures and are highly adaptable. Furthermore, it is also a vector for Aphanomyces astaci, causing crayfish plague. Due to its high adaptability, fast growth and reproduction, since one individual is enough to start a new population, it is important to understand its invasion ecology in order to implement better management plan in the new invaded ecosystems. In this thesis the main goal was to assess whether the temperature is the main factor of marbled crayfish establishment and distribution in the artificially warm outflow channel of Balti Power Plant in Narva. We hypothesised to find temperature gradient along the channel which reflects marbled crayfish distribution and trophic niche. We analysed marbled crayfish and their potential food sources for stable carbon (13C) and nitrogen (15N) isotopes to assess its trophic niche. Temperature data did not show the gradient in the channel, however the channel was significantly warmer than Narva Reservoir. Stable isotope results showed change in marbled crayfish diets and trophic niche along the channel and between seasons, indicating a shift from enriched carbon to depleted carbon values from head to mouth of the channel and a shift from high to lower trophic level from spring and summer to autumn. Moreover, results showed diets being similar in head and middle of channel where crayfish mostly rely on macroinvertebrates and macrophytes, while in the mouth of the channel diet seems to shift more towards periphyton. Diet also changed from protein-rich in spring to vegetation-based diet in autumn. Based on the results, temperature had important role in the marbled crayfish establishment in the invaded channel, however the distribution of its population might have been affected by ecological aspects rather than temperature, like better food availability in the head of the channel.

 

Keywords: invasive species • trophic niche • temperature • Procambarus virginalis • stable isotopes


Note: Embargoed until 3 September 2021.

27 May 2021

Marmorkrebs in Taipei update

Last November, I reported on Marmorkrebs were found in Taipei, but they seemed to be confined to a single park pond. A brief mention in a new story suggests there is a second location that has become home to a population of Marmorkrebs. 


The firefly restoration ecological pond in Taipei’s Daan Forest Park is being invaded by an exotic species, the many-rayed sailfin sucker catfish, while Neihu District’s (內湖) Bihu Park is being invaded by the invasive marbled crayfish, causing ecological balance issues, the office said.

 

Emphasis added.

 

Marmorkrebs were previously reported in Daan Forest Park, so it is not entirely clear to me if Marmorkrebs are in two parks, or id the reporter inadvertently switched the two locations. I have updated the map of Marmorkrebs introductions with the second Taipei location, but noted the uncertainty.

 

External links


Taipei to issue fines for feeding wildlife in parks

 

Related posts

 

Marmorkrebs’ second Asian invasion

14 May 2021

Roje and colleagues 2021

Roje S, Richter L, Worischka S, Let M, Veselý L, Buřič M. 2021. Round goby versus marbled crayfish: Alien invasive predators and competitors. Knowledge and Management of Aquatic Ecosystems 422: 18. https://doi.org/10.1051/kmae/2021019

 

Abstract


Aquatic biodiversity is threatened by spread of invasive alien species. Round goby Neogobius melanostomus is an invasive fish in large European rivers as well as in coastal waters near their mouths and marbled crayfish Procambarus virginalis is a highly invasive crustacean. Both are small, bottom-dwelling species occupying similar habitat and shelters and utilizing similar food sources. We hypothesized that goby presents a threat to both native and non-native astacofauna in invaded ecosystems. We tested this through laboratory experiments designed to determine aggressiveness and competitiveness of goby against marbled crayfish as a model for other North American cambarid crayfish, assessing goby prey size selection and competition with marbled crayfish for space and shelter. Gobies showed high aggressiveness and dominance over the crayfish. Goby predation on juvenile crayfish was limited by mouth gape size. In goby/crayfish pairs of similar weight, gobies were more aggressive, although each affected the behavior of the other.

 

Keywords: Biological invasion • freshwater • predation • shelter competition • species interaction

 

Open access

 

11 May 2021

Kouba and colleagues 2021

Biology journal logo

Kouba A, Lipták B, Kubec J, Bláha M, Veselý L, Haubrock PJ, Oficialdegui FJ, Niksirat H, Patoka J, Buřič M. 2021. Survival, growth, and reproduction: Comparison of marbled crayfish with four prominent crayfish invaders. Biology 10(5): 422. https://www.mdpi.com/2079-7737/10/5/422

 

Abstract


Biological invasions are increasingly recognized ecological and economic threats to biodiversity and are projected to increase in the future. Introduced freshwater crayfish in particular are protruding invaders, exerting tremendous impacts on native biodiversity and ecosystem functioning, as exemplified by the North American spiny-cheek, signal and red swamp crayfish as well as the Australian common yabby. The marbled crayfish is among the most outstanding freshwater crayfish invaders due to its parthenogenetic reproduction combined with early maturation and high fecundity. As their introduced ranges expand, their sympatric populations become more frequent. The question of which species and under what circumstances will dominate in their introduced communities is of great interest to biodiversity conservation as it can offer valuable insights for understanding and prioritization of management efforts. In order to examine which of the aforementioned species may be more successful as an invader, we conducted a set of independent trials evaluating survival, growth, claw injury, and reproduction using single-species stocks (intraspecific interactions) and mixed stocks (interspecific interactions) of marbled crayfish vs. other crayfish invaders since the onset of exogenous feeding. In both single and mixed stocks, red swamp crayfish and yabby grew faster than marbled crayfish, while marbled crayfish were superior to both spiny-cheek and signal crayfish in terms of growth. With the exception of signal crayfish, the faster-growing species consistently reached a higher survival rate. The faster-growing species tended to negatively impair smaller counterparts by greater claw injury, delayed maturation, and reduced fecundity. Only marbled crayfish laid eggs as early as 14 weeks in this study, which is earlier than previously reported in the literature. Thus, the success of marbled crayfish among invasive crayfish is significantly driven by relatively fast growth as well as an early and frequent reproduction. These results shed light on how interactions between invasive populations can unfold when their expansion ranges overlap in the wild, thereby contributing to the knowledge base on the complex population dynamics between existing and emerging invasive species. 


Keywords: Biological invasion • pet trade • animal release • species interactions • sympatry


Open access


30 April 2021

Zoobabies, 2021 edition

The Cincinatti Enquirer answers a question I’ve been wondering for a while. 

 

Some years ago, I noted the Cincinatti Zoo had a display of “Marbled crayfish” and listed them as Procambarus fallax. The zoo’s description mentioned nothing about asexual reproduction, so I thought they were slough crayfish from Florida or Georgia.


But a new article about the zoo says:

 

This baby marbled crayfish resembles a small lobster, but it will only grow to 2 to 3 inches long. All marbled crayfish are women. They don't need a partner to reproduce.
 

Marmorkrebs they are, then! Which makes me wonder why their reproduction wasn’t mentioned on the display.


Related posts

 

Zoo babies

 

External links

 

Up close with the Cincinnati Zoo's most adorable ambassadors

29 April 2021

International Conference on Aquatic Invasive Species 2022

International Conference on Aquatic Invasive Species logo

The date for the next conference on aquatic invasive species will be 18-22 April 2022 in Belgium. It will be a mix of in person and online and the focus will be on climate change. But I’m sure there will be some crayfish content!

 

It must be challenging to plan conferences, since COVID-19 is still out of control in some regions but maybe coming under control with vaccination and we really don’t know what will happen.


External links

 

22nd International Conference on Aquatic Invasive Species


22 April 2021

Ontario seeks public comment on regulating marbled crayfish

Ontario Ministry of Natural Resources

Nation Valley News reports that Ontario is moving to the next phase of a process that could result in the regulation of Marmorkrebs in the province, along with twelve other aquatic species.

 

The first round of consultation identified “no significant concerns” of regulating Marmorkrebs.

 

A ban on Marmorkrebs in Ontario would arguably be the most significant regulatory action in North America yet implemented. Ontario is Canada’s most populous province. It’s also one of the largest single jurisdictions in terms of size in North America. Currently, Marmorkrebs are routinely available in the pet trade.


Louisiana red swamp crayfish (Procambarus clarkii) are also on the list. This crayfish would get an exception for its use as food.


Comment is open until 7 June 2021.


External links

 

Regulating 13 invasive species and watercraft as a carrier of invasive species under Ontario’s Invasive Species Act, 2015

 

Seeking information on invasive species and carriers under the Ontario Invasive Species Act, 2015 (closed)

 

Province seeks input into wild pig strategy and updates to Invasive Species Act
 

19 April 2021

Scheers and colleagues 2021

BioInvasions Records cover.

Scheers K, Brys R, Abeel T, Halfmaerten D, Neyrinck S, Adriaens T. 2021. The invasive parthenogenetic marbled crayfish Procambarus virginalis Lyko, 2017 gets foothold in Belgium. BioInvasions Records 10(2): 326-340. https://doi.org/10.3391/bir.2021.10.2.11

 

Abstract

 

In 2020, four populations of the marbled crayfish Procambarus virginalis, which is included on the list of Invasive Alien Species of Union concern, were discovered in northern Belgium. These records represent the first established populations of this invasive parthenogenetic species in the Benelux. The marbled crayfish seems well established at all sites where it was discovered. Genetic analysis confirmed the species’ identity with the obtained COI Folmer fragments being 100 percent identical to reference sequences of P. virginalis from Germany, Italy, Sweden and the Czech Republic. We proposed a single diagnostic nucleotide for unambiguous character-based species identification between P. virginalis and P. fallax. The finding of this new species through opportunistic surveys instigated by citizen science reports indicates considerable knowledge gaps on crayfish distribution in Belgium. Considering the regulated status of most species in Belgium, we advocated the further set-up of dedicated crayfish surveillance using passive and active monitoring including environmental DNA detection.

 

Keywords: invasive species, Cambaridae, non-native species, Procambarus fallax forma virginalis


Open access


Stara and colleagues 2021

Chemosphere cover

Stara A, Zuskova E, Vesely L, Kouba A, Velisek J. 2021. Single and combined effects of thiacloprid concentration, exposure duration, and water temperature on marbled crayfish Procambarus virginalis. Chemosphere 273: 128463. https://doi.org/10.1016/j.chemosphere.2020.128463


Abstract


The increasing utilization of chemicals and ongoing climate change have a negative impact on aquatic ecosystems. The present study examined combined effects of water temperature, chemical concentration, and duration of exposure to the neonicotinoid thiacloprid on marbled crayfish Procambarus virginalis. Crayfish were exposed to thiacloprid at the environmental concentration of 4.50 μg L-1 and 10% 96LC50 to marbled crayfish, 64.64 μg L-1, at water temperature of 17 and 23°C for 28 days followed by a 28 day depuration period. No crayfish died during the experiment. Both thiacloprid concentrations at 23°C showed a synergistic effect with temperature on the biochemical indicators in haemolymph compared to those at 17°C. Both concentrations of thiacloprid at both temperatures were associated with significant differences from thiacloprid-free controls (P < 0.01) in haemolymph glucose, ammonia, calcium, inorganic phosphate, and lactate; haemolymph enzymes aspartate aminotransferase, alanine aminotransferase, creatine kinase, and alkaline phosphatase; antioxidant biomarkers superoxide dismutase, catalase, glutathione S-transferase, and reduced glutathione in hepatopancreas, muscle, and gill, and showed lipid peroxidation in hepatopancreas and muscle. Histological analyses revealed structural changes and damage to gill and hepatopancreas of exposed crayfish.


Keywords: neonicotinoid • insecticide • non-target organism • synergism • haemolymph • antioxidants

Grandjean and colleagues 2021

BioInvasions Records cover.

Grandjean F, Collas M, Uriarte M, Rousset M. 2021. First record of a marbled crayfish Procambarus virginalis (Lyko, 2017) population in France. BioInvasions Records 10(2): 341-347. https://doi.org/10.3391/bir.2021.10.2.12

 

Abstract

 

Here we report the first record of marbled crayfish Procambarus virginalis in France. In total, 34 individuals were found in a pond close to the Moselle River, coexisting with the invasive Faxonius limosus and the native Astacus astacus. Their presence seems limited to one pond of the seven located in this area, probably due to a very recent human introduction. Molecular analyses based on COI mt gene confirmed the morphological identification of captured specimens as marbled crayfish. The risk of spread to the Moselle is very high particularly during flood periods. Management recommendations are given. 

 

Keywords: invasive species • parthenogenetic crayfish • coexisting crayfish species • mitochondrial DNA


Open access


18 April 2021

Ohio lists Marmorkrebs as “Injurious aquatic invasive species”

Map of United States with Ohio highlighted.

Ohio is the latest North American jurisdiction to regulate Marmorkrebs.

 

In a PDF dated 9 April 2021 in the file name, Marmorkrebs is designated an Injurious Aquatic Invasive Species (IAIS). The one page pamphlet notes:

 

Listed species are unlawful to possess, import, or sell unless dead and/or preserved.

However, the Department of Natural Resources list of injurious aquatic invasive species is on a page that says “Published on Jul 31, 2020” in the search results. So it’s possible that Marmorkrebs were already listed last year and the new document is just the start of publicizing the new regulation. Another PDF from Spring 2019 indicates that Ohio was planning to add marbled crayfish to the list. But it hadn’t happened yet.


It’s symptomatic of a recurring problem. I, as someone who actually cares about when new regulations about this species are passed, cannot find out when these things happen. What chance does a normal pet owner with a 10 gallon aquarium have?


External links

 

Marbled Crayfish (Marmorkrebs) Control in Ohio (PDF)

Injurious Aquatic Invasive Species


 

09 April 2021

New website: The Perfect Invader

The Perfect Invader logo

The Perfect Invader is a new project to examine how the introduction of Marmorkrebs in Madagascar is affecting human health. 

 

Part of the project is looking at how people are eating Marmorkrebs. Some of that has already been published (e.g., here and here).

 

A newer aspect of the project, which I’ve seen in relation to other crayfish, but not Marmorkrebs, is how the crayfish affect schistosomiasis infections.

 

Check it out!

 

External links

 

The Perfect Invader

 

17 March 2021

Okada and colleagues 2021

The Journal of Experimental Biology cover

Okada S, Hirano N, Abe T, Nagayama T. 2021. Aversive operant conditioning alters the phototactic orientation of the marbled crayfish. The Journal of Experimental Biology 224(6): jeb242180. https://doi.org/10.1242/jeb.242180

 

Abstract 

 

Aversive learning was applied to affect the phototactic behaviour of the marbled crayfish. Animals initially showed negative phototaxis to white light and positive taxis to blue light. Using an aversive learning paradigm, we investigated the plasticity of innate behaviour following operant conditioning. The initial rate of choosing a blue-lit exit was analysed by a dual choice experiment between blue-lit and white-lit exits in pre-test conditions. During training, electrical shocks were applied to the animals when they oriented to the blue-lit exit. Memory tests were given to analyse the orientation rate to the blue-lit exit in trials 1 h and 24 h after training and these rates were compared with the pre-test. In general, animals avoided the blue-lit exit in the memory tests. When training was done three times, the long-term memory was retained for at least 48 h, although a single bout of training was also enough to form a long-term memory. Cooling animals at 4 °C or injection of cycloheximide immediately after training altered the formation of long-term memory, but had no effect on short-term memory formation. Administration of the adenylate cyclase inhibitor SQ22536, the PKA inhibitor H89, or the CREB inhibitor KG-501 immediately after training also blocked the formation of long-term memory, but had no effect on short-term memory formation. Thus, our pharmacological behavioural analyses showed that new protein synthesis was necessary to form long-term memories and that the cAMP/PKA/CREB pathway is the main signal cascade for long-term memory formation in the marbled crayfish.

 

Keywords: None provided.

26 February 2021

Heroes release zeroes

The Illinois-Indiana Sea Grant has started a new campaign against invasive species. The aquatic species arm of the campaign is Release Zero.

 

I particularly appreciate the point that it’s hard to be a responsible pet owner when so many providers don’t know or provide scientific names. There are hundreds of species of crayfish with different invasive potential, so just selling “crayfish” is irresponsible. There is a great need for quick, high quality ways of identifying species in the pet trade.

 

Marmorkrebs are not featured on the main webpage, but are species of concern for the Great Lakes, as the project’s Twitter account points out.

 

Wild marbled crayfish populations are established across Europe and elsewhere. They have NOT been found in the Great Lakes! 🙌 Aquarium owners, anglers and others can help keep it that way.


We need way more education on this, so I hope this campaign is very successful!


External links

 

Release Zero website

24 February 2021

Marmorkrebs talk online, 26 February 2021

Seminar announcement poster featuring Saisupritha Talasu is presenting “Identifying various biogenic amine receptors in Procambarus virginalis”

Wolfgang Stein announced a seminar about Marmorkrebs will be held on Zoom!

Saisupritha Talasu is presenting “Identifying various biogenic amine receptors in Procambarus virginalis” at 12:00 pm (noon) Central time on Friday, 26 February 2021. The Zoom meeting ID is 630 494 073.

It will be followed by a talk on escape responses, species unknown.

19 February 2021

Invasive Species Week is coming...

 Mark your calendars for 24 May, 2021. That will be the start of Invasive Species Week.


Invasive Species Week, 24-30 May 2021.

This blog will be participating, naturally!


External links

 

Invasive Species Week

15 February 2021

Wen 2020

Swedish University of Agricultural Sciences logo
Wen Y. 2020. Characterization of MITF gene in crayfish and their possible role in innate immunity. Master’s thesis, Department of Animal Breeding and Genetics, Sveriges lantbruksuniversitet (Swedish University of Agricultural Sciences). http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-16418

Abstract

Microphthalmia-associated transcription factor (MITF) is a tissue-specific transcription factor (TF), with a basic helix-loop-helix leucine zipper (bHLH-LZ) domain, which binds to the canonical E-box sequence (5’-CANNTG-3’) in the promoter region of target genes. In this study, the MITF-like protein was identified in the marbled crayfish (Procambarus virginalis). The full-length cDNA coding sequence of the most similar gene to MITF from marbled crayfish is 1284bp (427 amino acids). In the secondary and tertiary structure of the deduced amino acid sequence, a conserved functional structure of bHLH-LZ was shown, which could bind to E-box. In the phylogenetic analysis, this obtained MITF-like gene showed a lager evolutionary distance to all the vertebrates and some invertebrates like lancelet (Branchiostoma belcheri), starfish (Acanthaster planci), sea urchin (Paracentrotus lividus) and (Lytechinus variegatus), but is closer than worms (Caenorhabditis elegans). The bHLH-LZ domain is located in exon 6, exon 7, exon 8, and exon 9 in the MITF gene and has a similar structure as the corresponding exons in human. Besides, in this study, a comparison of exon-intron structure of MITF between human, mouse, fruit fly and marbled crayfish was performed, it was shown that the splicing sites of the bHLH-LZ domain in MITF gene might be conserved across species. To evaluate the possible role of MITF as a TF in innate immune system regulation, a similar prophenoloxidase (proPO) DNA sequence was analysed for the presence of E-box. The proPO gene is responsible for trapping and myelinization of pathogens in invertebrate. The proPO-like gene of marbled crayfish contains eight CANNTG sequences. In addition, anti-apoptotic factor (BCL-2)-like gene was found in marbled crayfish and four CANNTG sequences were found. Our results provided evidence of the presence of MITF-like gene in crayfish species and may provide knowledge on the role of MITF in innate immune activation.

Keywords: Microphthalmia-associated transcription factor • basic Helix-Loop-Helix Leucine Zipper • crayfish • Aphanomyces astaci • immunity

12 February 2021

Marmorkrebs continue advance in Asia

Map of China showing Macao.
Marmorkrebs have been found near the mainland of the People’s Republic of China, according to the Macao Daily Times.


The news article is short on details, like how many crayfish or exactly where they were found, with the newspaper simply referring to a “leisure area” on Taipa in Macau. Taipa is technically an island, but sits so close to the mainland that it is connected by reclaimed land. 


The impact of Marmorkrebs in China could be immense. The Louisiana red swamp crayfish was embraced by the restaurant business and is now one of the most popular dishes in China, so there is clearly money to be made on crayfish.


But one of the interesting points of the article is that the concern about invasive species it lists is different than other countries. The article raises the fear that invasive will remove natives that are used for... traditional Chinese medicine.


The map of Marmorkrebs introductions has been updated.


Marmorkrebs.
Update: The Macau Post Daily has an article with a little more detail. It sounds like it was a single individual. It also gave a more precise location: “the ecological pond in the Camellia Garden.” Which, unfortunately, I cannot find on Google Maps yet.

 

There is no information up at the Municipal Affairs Bureau yet. At least not on the English site. 

 

More update: Ah, here’s the park with Camellia Garden. And this seems to be the person who found them.

 

External links


Foreign species of crayfish, hazardous to local ecology, found in Taipa

Invasive marbled crayfish found on Big Taipa Hill: IAM

10 February 2021

van Kuijk and colleagues 2021

Scientific Reports

van Kuijk T, Biesmeijer JC, van der Hoorn BB, Verdonschot, PFM. 2021. Functional traits explain crayfish invasive success in the Netherlands. Scientific Repprts 11: 2772. https://doi.org/10.1038/s41598-021-82302-4

 

Abstract

 

Biological invasions by nonindigenous species can have negative effects on economies and ecosystems. To limit this impact, current research on biological invasions uses functional traits to facilitate a mechanistic understanding of theoretical and applied questions. Here we aimed to assess the role of functional traits in the progression of crayfish species through different stages of invasion and determine the traits associated with invasive success. A dataset of thirteen functional traits of 15 species currently occurring or available for sale in the Netherlands was evaluated. Six of these crayfish appeared invasive. Important traits distinguishing successful from unsuccessful invaders were a temperate climate in the native range, a medium to high egg count and producing more than one egg clutch per year. The most successful invaders had different functional trait combinations: Procambarus clarkii has a higher reproductive output, can migrate over longer distances and possesses a higher aggression level; Faxonius limosus is adapted to a colder climate, can reproduce parthenogetically and has broader environmental tolerances. Using a suit of functional traits to analyse invasive potential can help risk management and prevention. For example, based on our data Procambarus virginalis is predicted to become the next successful invasive crayfish in the Netherlands.

 

Keywords: None provided.


Open access


04 February 2021

Veselý and colleagues 2021

Cover of Hydrobiologia, volume 848, issue 3.
Veselý L, Ruokonen TJ, Weiperth A, Kubec J, Szajbert B, Guo W, Ercoli F, Bláha M, Buřič M, Hämäläinen H, Kouba A. 2021. Trophic niches of three sympatric invasive crayfish of EU concern. Hydrobiologia 848(3): 727737. https://doi.org/10.1007/s10750-020-04479-5

 

Abstract

 

The spread of non-native species results in novel and often unexpected assemblages. Using stable isotopes, we disentangled the trophic relationships between three invasive crayfish species at two sites of a small thermal tributary of the Barát stream, Hungary. We studied Procambarus virginalis and Faxonius limosus living in sympatry in the upper section of this thermal tributary, and then an assemblage in a lower section also containing P. clarkii. The two species in the upper section largely shared trophic niches, although P. virginalis was more carnivorous than F. limosus, which fed more on detritus and aquatic plants. In the lower section, P. clarkii had a distinctive trophic niche, being more carnivorous than the other species and also preying on other crayfish and fish. The trophic niches of the other two species shifted slightly, being narrower and more overlapping in the presence of P. clarkii. It seems that the presence of P. clarkii affects the feeding habits and trophic niches of the other two crayfish. Our results also indicate that the species have somewhat distinctive feeding niches, which suggests that the ecosystem effects of these species are likely to be at least partially additive in the shared localities.

 

Keywords: biological invasion • Faxonius limosus • Procambarus virginalisProcambarus clarkii • sympatry

29 January 2021

Ranja Andriantsoa interview

Ranja Andriantsoa


Rowan Moore Gerety, author of the excellent Harper’s article on Marmorkrebs in Madagascar, continues his coverage of the story with another outstanding piece. This is an interview with Ranja Andriantsoa.


The article also has a nice video which is one of the best I’ve seen for giving a sense for just how abundant Marmorkrebs are in Madagascar.




External links

 

Invasion of the crayfish clones: Q&A with Ranja Andriantsoa

22 January 2021

Maiakovska and colleagues, 2021

Communications Biology logo.
Maiakovska O, Andriantsoa R, Tönges S, Legrand C, Gutekunst J, Hanna K, Pârvulescu L, Novitsky R, Weiperth A, Sciberras A, Deidun A, Ercoli F, Kouba A, Lyko F. 2021. Genome analysis of the monoclonal marbled crayfish reveals genetic separation over a short evolutionary timescale. Communications Biology 4(1): 74. https://doi.org/10.1038/s42003-020-01588-8

 

Abstract


The marbled crayfish (Procambarus virginalis) represents a very recently evolved parthenogenetic freshwater crayfish species that has invaded diverse habitats in Europe and in Madagascar. However, population genetic analyses have been hindered by the homogeneous genetic structure of the population and the lack of suitable tools for data analysis. We have used whole-genome sequencing to characterize reference specimens from various known wild populations. In parallel, we established a whole-genome sequencing data analysis pipeline for the population genetic analysis of nearly monoclonal genomes. Our results provide evidence for systematic genetic differences between geographically separated populations and illustrate the emerging differentiation of the marbled crayfish genome. We also used mark-recapture population size estimation in combination with genetic data to model the growth pattern of marbled crayfish populations. Our findings uncover evolutionary dynamics in the marbled crayfish genome over a very short evolutionary timescale and identify the rapid growth of marbled crayfish populations as an important factor for ecological monitoring.

 

Keywords: None provided. 


Open access


Additional: Blog post by two of the authors about this paper:

Tönges S, Lyko F. 2021. Population growth and utilization of clonal marbled crayfish. Nature Ecology and Evolution. https://natureecoevocommunity.nature.com/posts/population-growth-and-utilization-of-clonal-marbled-crayfish-18289693-c2e2-4213-b624-26c114b5b428 2021.

19 January 2021

Harper’s magazine features Marmorkrebs in Madagscar

Harper's February 2021 cover
Rowan Moore Gerety has just published the best article ever written about Marmorkrebs.

 

“Stowaways” appears in the February 2021 issue of Harper’s magazine. It’s a feature-length examination of the invasion of Marmorkrebs on Madagascar. It was prompted by conversation with Julia Jones and was almost two years in the making.

 

There is some science, but the bulk of the article is a nuanced, detailed examination of the complexities of living with environmental change.

 

Over and over again, people are ambivalent to Marmorkrebs. People recognize the crayfish is a pest... but it’s a cheap meal. In a place where most people are living on the equivalant of a couple of US dollars a day, that matters.


In the hills above town, where rice cultivation depends on rainfall for irrigation, farmers complain that the levees of their terraced paddies spring leaks without warning, sparking arguments when valuable rainwater drains into their neighbors’ fields instead. Most realize that crayfish burrows are to blame, but suspicions linger all the same. “The tsi pe’peo is an enemy of agriculture,” Ramandrosoa told me. Nevertheless, he hesitated to advocate for its eradication. For families without even enough money to pay their children’s school fees, he said, “It’s kerosene, it’s sugar, it’s soap. It’s the basic necessities.” In the lean months, Ramandrosoa explained, “People collect crayfish so they don’t have to sell their rice.”


I also love the illustrations by Barry Falls. Look at this gem:


Illustration of marbled crayfish overlaying a map of Madagascar

I’ll forgive that the abdomen probably can’t bend quite that much.

 

I will probably be rereading this many times. It’s a rich and insightful part of a story that I know from the published literature, but this shows just how little of the story makes it into journal articles.

 

Highly recommended.


External links


Gerety RM. 2021. Stowaways. Harper’s 342(2049): 72-79. https://harpers.org/archive/2021/02/stowaways-crayfish-madagascar/

04 January 2021

Stein and colleagues, 2020

The Journal of Undergraduate Neuroscience Education logo
Stein W, Talasu S, Vidal-Gadea A, DeMaegd ML. 2020. Physiologists turned Geneticists: Identifying transcripts and genes for neuronal function in the marbled crayfish, Procambarus virginalis. The Journal of Undergraduate Neuroscience Education 19(1): A36-A51. https://www.funjournal.org/wp-content/uploads/2020/11/june-19-36.pdf

 

Abstract

 

The number of undergraduate researchers interested in pursuing neurophysiological research exceeds the research laboratory positions and hands-on course experiences available because these types of experiments often require extensive experience or expensive equipment. In contrast, genetic and molecular tools can more easily incorporate undergraduates with less time or training. With the explosion of newly sequenced genomes and transcriptomes, there is a large pool of untapped molecular and genetic information which would greatly inform neurophysiological processes. Classically trained neurophysiologists often struggle to make use of newly available genetic information for themselves and their trainees, despite the clear advantage of combining genetic and physiological techniques. This is particularly prevalent among researchers working with organisms that historically had no or only few genetic tools available. Combining these two fields will expose undergraduates to a greater variety of research approaches, concepts, and hands-on experiences. The goal of this manuscript is to provide an easily understandable and reproducible workflow that can be applied in both lab and classroom settings to identify genes involved in neuronal function. We outline clear learning objectives that can be acquired by following our workflow and assessed by peer-evaluation. Using our workflow, we identify and validate the sequence of two new Gamma Aminobutyric Acid A (GABAA) receptor subunit homologs in the recently published genome and transcriptome of the marbled crayfish, Procambarus virginalis. Altogether, this allows undergraduate students to apply their knowledge of the processes of gene expression to functional neuronal outcomes. It also provides them with opportunities to contribute significantly to physiological research, thereby exposing them to interdisciplinary approaches.

 

Keywords: undergraduate • peer-mentoring • GABA • Marmorkrebs • neurophysiology • gene annotation • decapod • crustacean

 

Open access