Jurmalietis and colleagues 2024

Jurmalietis R, Grickus A, Elstina A. 2024. Marbled crayfish (Procambarus virginalis) as a promising object for aquaculture industry. Conferences of Rezekne Academy of Technologies, Environment. Technology. Resources. 12th International Scientific and Practical Conference. June 27-28, 2024, “'Vasil Levski”' National Military University, Veliko Tarnovo, Bulgaria. https://conferences.ru.lv/index.php/ETR/ETR2019/rt/printerFriendly/3287/0

Abstract

Marbled crayfish / marmorkrebs, parthenogenetically reproducing decapod crustacean of pet aquarium origin conventionally has been recognized as an undesirable species since it a) is an invasive pest dangerous for aquatic ecosystem resources because of its hardiness, omnivorousness, fast growth, self-cloning abilities; b) has little commercial value for food industry. However, recently an idea has been introduced about marmorkrebs as a promising cheap protein source, which can help to fight world hunger. Accordingly, research performed at Liepaja University (Latvia) was focused on marbled crayfish farming system design issues. Research paper presented discusses marmorkrebs survival capacities in small-volume microcosm tanks (made from recycled material), under harsh abiotic conditions. Pilot research results obtained will be used to develop experimental project on low-input microcosmic indoor aquaculture for marmorkrebs. 

Keywords: None provided.

Scheers 2023

Scheers K. 2023. The illegal trade of marbled crayfish in Belgium: an ongoing risk for further spread. Presentation given to CrayfIT 2023 conference. https://crayfit.eu/wp-content/uploads/2023/09/Crayfit-Book-of-Abstracts.pdf

Abstract

The marbled crayfish (Procambarus virginalis) is a well-known invasive species. This parthenogenetic species originated from the aquarium trade and has been introduced in many European countries. Because of its highly invasive character this species was included on the list of Invasive Species of Union Concern in 2016 because of its potential as a highly invasive species. This resulted in a total ban of the trade, breeding and keeping of marbled crayfish in the European Union. To ascertain if marbled crayfish are still being traded in Belgium, the major online classified platforms were regularly monitored from 26-04-2022 to 01-08-2023 for the sale of the banned marbled crayfish. The results of this survey show that the marbled crayfish is still commonly being traded online and indicate the high risk of further spread.

 

Keywords: marbled crayfish • Procambarus virginalis • trade • legislation • enforcement

Kaur and colleagues 2023

Kaur D, Iqbal A, Soto I, Kubec J, Buřič M. 2023. Effects of chemical cues and prior experience on predator avoidance in crayfish. Presentation given to CrayfIT 2023 conference. https://crayfit.eu/wp-content/uploads/2023/09/Crayfit-Book-of-Abstracts.pdf

Abstract

Multisensory stimuli provide organisms with information to assess the threat present in the surroundings. The impact of the chemical predatory cues combined with experiences gained between species previously unknown to each other was not fully understood. We investigated the foraging and behaviour of naïve and experienced marbled crayfish (Procambarus virginalis) juveniles in response to the chemical signals of pumpkinseed (Lepomis gibbosus) alone, and during the active foraging on the marbled crayfish juveniles. Naïve and experienced crayfish juveniles were exposed to three different conditions: the water inlet came from 1) a tank with predator actively foraging on their conspecifics, 2) the a from tank with predator only, and 3) from an empty tank. Crayfish behaviour in arenas with food and shelter was video recorded for 4 hours. The positions of crayfish in specific zones in the arena (inlet, transition, and shelter zone) were localized every five minutes. Feed consumed was measured after the trial. The results obtained indicated that crayfish exposed to the combined stimuli avoided the inlet zone and preferred the shelter zone of the arena to a greater extent than did those crayfish exposed to predator-only cues and the control. Regardless of the stimuli, experienced crayfish showed significantly reduced foraging. Crayfish juveniles were more cautious because of previous experience rather than due to scent perception. Our findings imply that crayfish respond to threat-associated
odours with the greatest potency when visual or tactile cues are present, but previous experience with predator can cause high precaution.

Keywords: predation • crayfish • fish • kairomones • alarm cues

Stein and colleagues 2022

Stein W, DeMaegd ML, Benson AM, Roy RS, Vidal-Gadea AG. 2022. Combining old and new tricks: The study of genes, neurons, and behavior in crayfish. Frontiers in Physiology 13: 947598. https://doi.org/10.3389/fphys.2022.947598

 Abstract

For over a century the nervous system of decapod crustaceans has been a workhorse for the neurobiology community. Many fundamental discoveries including the identification of electrical and inhibitory synapses, lateral and pre-synaptic inhibition, and the Na+/K+-pump were made using lobsters, crabs, or crayfish. Key among many advantages of crustaceans for neurobiological research is the unique access to large, accessible, and identifiable neurons, and the many distinct and complex behaviors that can be observed in lab settings. Despite these advantages, recent decades have seen work on crustaceans hindered by the lack of molecular and genetic tools required for unveiling the cellular processes contributing to neurophysiology and behavior. In this perspective paper, we argue that the recently sequenced marbled crayfish, Procambarus virginalis, is suited to become a genetic model system for crustacean neuroscience. P. virginalis are parthenogenetic and produce genetically identical offspring, suggesting that germline transformation creates transgenic animal strains that are easy to maintain across generations. Like other decapod crustaceans, marbled crayfish possess large neurons in well-studied circuits such as the giant tail flip neurons and central pattern generating neurons in the stomatogastric ganglion. We provide initial data demonstrating that marbled crayfish neurons are accessible through standard physiological and molecular techniques, including single-cell electrophysiology, gene expression measurements, and RNA-interference. We discuss progress in CRISPR-mediated manipulations of the germline to knock-out target genes using the ‘Receptor-mediated ovary transduction of cargo’ (ReMOT) method. Finally, we consider the impact these approaches will have for neurophysiology research in decapod crustaceans and more broadly across invertebrates.

 

Keywords: marbled crayfish • CRISPR • tail flip • stomatogastric ganglion • marmorkrebs • giant fiber • RNAi • transgenesis

Steen and colleagues 2023

Steen F, Scheers K, De Knijf G. 2023. Controlling Marbled Crayfish in an urban pond: a dual approach involving intensive trapping and predator introduction. Presentation given to CrayfIT 2023 conference. https://crayfit.eu/wp-content/uploads/2023/09/Crayfit-Book-of-Abstracts.pdf

Abstract

The marbled crayfish is a newly introduced invasive species in the Flanders region of Belgium, first sighted in 2017. The species has primarily been sighted in artificial ponds in urbanised areas, suggesting its introduction through the release of aquarium pets. Marbled Crayfish have not yet spread to open water systems in Belgium, presenting an opportunity to experiment with control measures within a closed system. To manage this species within the urban park pond in the municipality of Westende, a pilot study was developed. The municipality did not approve of draining the pond, and the use of chemical agents is prohibited in aquatic environments in Flanders. To comply with these restrictions, we developed an alternative management measure to decrease crayfish densities and minimise the risk of spread. A dual approach will be implemented, involving an intensive catch event followed by the introduction of two fish species, Cyprinus carpio and Lota lota to further suppress the population. Electrofishing and baited crayfish traps will be used to achieve a proportional reduction in densities at the start of the reproductive season. The introduction of fish is aims at preventing juvenile crayfish from reaching the reproductive phase, by creating a year-round predation pressure with common carp predating from spring to autumn, and burbot throughout the winter. This dual approach will be tested to
determine its effectiveness in controlling marbled crayfish densities. Preliminary results obtained after the first two phases of the study, namely population reduction and the introduction of the common carp, will be presented.

Keywords: Marbled Crayfish, invasive species management

Ložek and Vojs Staňová 2023

Ložek F, Vojs Staňová A. 2023. A rare observation of conjoined twins in marbled crayfish Procambarus virginalis. Presentation given to CrayfIT 2023 conference. https://crayfit.eu/wp-content/uploads/2023/09/Crayfit-Book-of-Abstracts.pdf

Abstract

Conjoined twins in crustaceans, including crayfish, have been predominantly observed during early developmental stages. However, cases of conjoined twins surviving into juvenile stages are infrequent and often associated with mortality resulting from complications during moulting due to morphological abnormalities. In this study, we present a unique case of marbled crayfish conjoined twins that remained viable for 36 days post-hatch, progressing into the 3^rd juvenile stage with the ability to moult. The fused individuals exhibited separate complete anteroposterior body axes, conjoined along the dorsal side of the cephalothorax. Both twins demonstrated successful predation of living nauplii of Artemia salina, their provided food source. Nonetheless, reduced mobility led to the growth of epibionts on their body surface, ultimately hindering the moulting process. This exceptional case sheds light on the prolonged survival and functional capabilities of conjoined twins in crayfish, expanding our understanding of their developmental biology.

Keywords: decapods, developmental aberration, parthenogenesis, genetic anomalies

Teesalu and colleagues 2023

Teesalu P, Maria-Muuga J, Hurt M, Kaldre K, Nõges T, Ercoli F. 2023. The role of temperature in marbled crayfish (Procambarus virginalis, Lyko 2017) invasion in Estonian freshwater ecosystem. Presentation given to CrayfIT 2023 conference. https://crayfit.eu/wp-content/uploads/2023/09/Crayfit-Book-of-Abstracts.pdf. Slide deck: https://dspace.emu.ee/server/api/core/bitstreams/9c9a5f04-fffc-4e21-b450-7c8b22650b94/content

Abstract

Marbled crayfish (Procambarus virginalis) is an example of a freshwater invader spreading
across Europe, and understanding its establishment and distribution is crucial. This study
aimed to assess the role of temperature in the invasion of marbled crayfish in the artificially
warm outflow channel of a power plant in Estonia. It was hypothesized that warmer water
temperature and the temperature gradient along the channel would impact crayfish
establishment, distribution, and trophic niche. Temperature loggers recorded a gradient,
revealing that the channel was significantly warmer than the reservoir, providing a suitable
habitat for crayfish. Stable isotope analyses showed spatial and seasonal trophic niche
shifts. The crayfish diet at the channel head mainly consisted of macroinvertebrates and
macrophytes, while the mouth had a diet dominated by periphyton. Additionally, the
crayfish shifted from consuming primary consumers in spring to primary producers in
autumn. Temperature played a crucial role in crayfish establishment, but other factors like
food source availability likely influenced population distribution. Overall, this research
sheds light on the importance of temperature and ecological aspects in the invasion
dynamics of marbled crayfish.

 

Keywords: stable isotopes • trophic niche • invasive species • population dynamic •
temperature effect