14 April 2015

#CloneClub

Last month, I finally manage to watch a show for which I’d heard many positive things:Orphan Black.

If you have not see it, I highly recommend this show. I’m writing about it here on this blog because the show is about genetically identical females – much like Marmorkrebs.


There are so many striking things about this show. The performance of lead actress Tatiana Maslany is astonishing. But as a biologist, I was struck by how sophisticated the portrayal of the science is. The show is fortunate to have a very good scientific consultant, Cosima Herter, who shares a first name with one of the lead characters.

The show’s take on clones stands in a stark contrast to many other depictions of clones in pop culture. Whereas most stories emphasize the similarities of the clones, Orphan Black runs the opposite way, and hammers away at the differences of the women in the show. The individual characterization is so complete and so well thought through and so consistent that you continually forget that it is all performed by one person. (Two if you count the body double Kathryn Alexandre).

It reminded me of my post from several years ago about how one of the great things about Marmorkrebs was that you could see the differences between sisters that started with the same identical genetic materials.

As I thought about it, another recent show also emphasized that clones were individuals: Star Wars: The Clone Wars series. As the series progressed, it gave the clone troopers names. Different haircuts. Different insignia. In short, the clones stopped being interchangeable cannon fodder and became distinct characters.


Do these shows reflect a larger cultural shift in our thinking about how genetics affects our identities? Too soon to tell, but I find the different portrayals of genetically identical individuals fascinating.

A new season of Orphan Black starts this weekend. And I can’t wait.

Related posts

How Marmorkrebs can make the world a better place

External links

Meet the real Cosima, Orphan Black's science consultant: The Crazy Science Of Orphan Black

The real life science behind Orphan Black
The many faces of Tatiana Maslany
Meet The Woman (Besides Tatiana Maslany) Who Plays Every Single "Orphan Black" Clone

07 April 2015

Marmorkrebs: the Early Years


Chris Lukhaup was one of the co-authors of the paper that introduced Marmorkrebs to the scientific world (Scholtz et al. 2003). He has been active in describing many new species of crustaceans, often emerging from the pet trade (for example, Lukhaup & Pekny 2006, 2008). He takes stunning pictures. He recently contacted me with this bit of history about Marmorkrebs, which I share with his permission (lightly edited):

When I contacted Jay Huner in Louisiana back in 2000 to tell him that I believe that the Marmorkrebs is a parthenogenetic species, he wrote me back and told me that this is impossible! I sent him some animals to check and he wrote me back that this was Procambarus clarkii. ;-) Also he told me that I need to look better because there have to be males and I should learn the difference between males and females.

In 2002, I had my first articles in some aquarium magazines describing the animal and warning already. I wrote an article and offered $3000 for a person bringing me a male Marmorkrebs... this was published in several magazines but nothing happened. Then I went to the USA myself to collect, and I was pretty sure that the Marmorkrebs was very close to Procambarus fallax. I send some animals to Berlin and they approved it. So this is the story of the Marmorkrebs from my side.

Thank you for providing that bit of history!

Reference

Lukhaup C, Pekny R. 2006. Cherax (Cherax) holthuisi, a new species of crayfish (Crustacea: Decapoda: Parastacidae) from the centre of the Vogelkop Peninsula in Irian Jaya (West New Guinea), Indonesia. Zoologische Mededelingen 80(1): 101-107. http://www.repository.naturalis.nl/document/41228

Lukhaup C, Pekny R. 2008. Cherax (Astaconephrops) boesemani, a new species of crayfish (Crustacea: Decapoda: Parastacidae) from the centre of the Vogelkop Peninsula in Irian Jaya (West New Guinea), Indonesia. Zoologische Mededelingen 82: 331-340. http://www.zoologischemededelingen.nl/82/nr02/a33


Scholtz G, Braband A, Tolley L, Reimann A, Mittmann B, Lukhaup C, Steuerwald F, Vogt G. 2003. Parthenogenesis in an outsider crayfish. Nature 421(6925): 806-806. http://dx.doi.org/10.1038/421806a

External links

Chris Lukhaup on Facebook
Chris Lukhaup’s pictures on Flickr

15 March 2015

Jirikowski and colleagues, 2015

Jirikowski G, Wolff C, Richter S. 2015. Evolution of eumalacostracan development--new insights into loss and reacquisition of larval stages revealed by heterochrony analysis. EvoDevo 6(1): 4. http://dx.doi.org/10.1186/2041-9139-6-4

Abstract

Background

Within Malacostraca (Crustacea), direct development and development through diverse forms of larvae are found. Recent investigations suggest that larva-related developmental features have undergone heterochronic evolution in Malacostraca. In the light of current phylogenetic hypotheses, the free-swimming nauplius larva was lost in the lineage leading to Malacostraca and evolved convergently in the malacostracan groups Dendrobranchiata and Euphausiacea. Here we reconstruct the evolutionary history of eumalacostracan (Malacostraca without Phyllocarida) development with regard to early appendage morphogenesis, muscle and central nervous system development, and determine the heterochronic transformations involved in changes of ontogenetic mode.

Results

Timing of 33 developmental events from the different tissues was analyzed for six eumalacostracan species (material for Euphausiacea was not available) and one outgroup, using a modified version of Parsimov-based genetic inference (PGi). Our results confirm previous suggestions that the event sequence of nauplius larva development is partly retained in embryogenesis of those species which do not develop such a larva. The ontogenetic mode involving a nauplius larva was likely replaced by direct development in the malacostracan stem lineage. Secondary evolution of the nauplius larva of Dendrobranchiata from this ancestral condition, involved only a very small number of heterochronies, despite the drastic change of life history. In the lineage leading to Peracarida, timing patterns of nauplius-related development were lost. Throughout eumalacostracan evolution, events related to epidermal and neural tissue development were clearly less affected by heterochrony than events related to muscle development.

Conclusions

Weak integration between mesodermal and ectodermal development may have allowed timing in muscle formation to be altered independently of ectodermal development. We conclude that heterochrony in muscle development played a crucial role in evolutionary loss and secondary evolution of a nauplius larva in Malacostraca.

Keywords: None provided.

25 February 2015

Faulkes, 2015

Faulkes Z. 2015. A bomb set to drop: parthenogenetic Marmorkrebs for sale in Ireland, a European location without non-indigenous crayfish. Management of Biological Invasions 6(1): 111-114. http://dx.doi.org/10.3391/mbi.2015.6.1.09

Abstract

Ireland is one of the few locations in Europe where non-indigenous North American crayfish species have not been introduced, and is a refuge for endangered white-clawed crayfish, Austropotamobius pallipes (Lereboullet, 1858). The parthenogenetic crayfish species Marmorkrebs, Procambarus fallax f. virginalis (Hagen, 1870), is sold in the pet trade in Ireland within the recorded range of A. pallipes. Marmorkrebs risk being introduced into Irish waters, where they could threaten A. pallipes populations, particularly as a vector for crayfish plague.

Keywords: Marmorkrebs • Procambarus fallax f. virginalisAustropotamobius pallipes • pet trade • Ireland • non-indigenous crayfish species

05 February 2015

The Crustacean Society / International Association of Astacology meeting in Australia, 2015


Oooh, this conference is like a superhero team-up!





Abstract submission is open for a joint meeting of The Crustacean Society (TCS) and the International Association of Astacology. They are looking for presentations from all fields of crustacean research worldwide and invite themed sessions and symposia.

Symposia suggestions should be on the symposium proposal form, and submitted by 14 March 2015.

Abstracts are to be according to the format outlined abstracts page of the conference web site, using the template. People are invited to submit abstracts on any aspect of crustacean research. Abstracts sunbmission deadline is 27 March 2015, and people will be notified of acceptance by 12 April 2015.

The meeting will be held at the Australian Museum. This museum, established in 1827, is the oldest museum in the country, with a long history of crustacean research and the most extensive crustacean collection in the Southern Hemisphere. The Museum is centrally located in the heart of Sydney City and is close to famous landmarks such as the Sydney Opera House and Harbour Bridge, the Royal Botanic Gardens, Art Gallery of New South Wales, the harbour, and hosts numerous tourist attractions, fine dining, and recreational activities.

External links

Conference webpage 

03 February 2015

Velisek and colleagues, 2014

Velisek J, Stara A, Koutnik D, Zuskova E, Kouba A. 2014. Effect of prometryne on early life stages of marbled crayfish (Procambarus fallax f. virginalis). Neuro Endocrinology Letters 35(Suppl 2): 93-98. http://www.ncbi.nlm.nih.gov/pubmed/25638372, http://europepmc.org/abstract/med/25638372

Abstract

OBJECTIVES: The aim of this study is to assess the toxicity of prometryne in early life stages of marbled crayfish (Procambarus fallax f. virginalis) on the basis of mortality, early ontogeny, growth rate, and histopathology during and at the end of the test.

DESIGN: The early life stages of marbled crayfish were exposed to prometryne at four concentrations, 0.51, (reported concentration in Czech rivers), 144, 1440, and 4320 µg.l-1 for 53 days and compared to crayfish in a non-treated control group.

RESULTS: Prometryne in concentration 144, 1444 and 4320 µg.l-1 caused decrease of weight and specific growth rates of crayfish. Crayfish exposed the highest concentration 4320 µg.l-1 showed delay in ontogeny development. All crayfish groups exposed to prometryne showed histopathological changes in gill. On the basis of histopathological changes the values of LOEC=0.51 µg.l-1 and NOEC=for 0.10 µg.l-1 of prometryne for marbled crayfish juveniles was estimated.

CONCLUSIONS: Chronic exposure of prometryne on early life stages of crayfish has affected their mortality, growth rate, and histology. Some of the changes were observed only at higher exposures (144, 1444 and 4320 µg.l-1), but histopathological changes in gills were observed also in crayfish exposed to the real environmental concentration in Czech rivers (i.e. 0.51 µg.l-1), which is about 9 times lower than maximal concentration (4.40 µg.l-1) reported in surface waters of Greece. Concentrations of prometryne in World rivers have been reported to generally vary in the range of 0.1-4.40 µg.l-1.

Keywords: None provided.

Note: As of 3 February 2015, the journal website has no papers past 2013. It is listed now because the full citation information is included in the PubMed entry. This entry will be updated when the PDF from the journal website becomes available.

Additional, 6 February 2015: The abstract is also listed in Europe PubMed Central.

Additional, 24 February 2015: The PDF of this paper is available on ResearchGate.

02 February 2015

Patoka and colleagues, 2015

Patoka J, Kalous L, Kopecký O. 2015. Imports of ornamental crayfish: the first decade from the Czech Republic’s perspective. Knowledge and Management of Aquatic Ecosystems 416: 04. http://dx.doi.org/10.1051/kmae/2014040

Abstract

The import of aquarium animals has been increasing worldwide in recent years. Despite its contribution to world trade and the economy, this trade also comprises one of the main pathways for the introduction of non-indigenous animals. In the past decade, crayfish has become a popular pet as well as a potential threat to the environment upon its escape or release. Since the Czech Republic is one of the world’s leading importer, exporter, and producer of aquatic ornamental animals, we prepared a detailed analysis of crayfish imports. The present paper provides a complete list of countries supplying ornamental crayfish and examines trends of their prices and imported quantities during the past decade (2003–2012). Indonesia has been identified as the leading supplier in recent years. The annual average price of imported crayfish has varied over the evaluated period within the range of €0.76–4.72 per individual and it is rising annually by €0.15. The quantity of live crayfish imported for aquarium purposes has not been affected significantly by the price per individual and it has grown rapidly. Therefore a constant monitoring of this pet trade sector is strongly recommended for the future.

Keywords: pet trade • aquarium • crustaceans • non-indigenous species • price