Faulkes Z, DeLeon H, Thomas J. 2018. Cloning crayfish cell culture. Poster presentation given at the International Crustacean Congress IX, 22-25 May 2018, Washington, DC, USA. http://www.birenheide.com/ICC2018/program/singlesession.php3?sessid=P, poster P.81.
Abstract
The parthenogenetic marbled crayfish, Marmorkrebs, is an emerging model organism. For example, it is the only decapod crustacean with a sequenced genome, and several labs have used Marmorkrebs as a model for embryonic development. One difficulty in studying embryonic cells is that eggs contain a large amount of yolk, which can make imaging embryonic cells difficult. We successfully isolated and cultured cells from early stage Marmorkrebs embryos, and confirmed their identity using DNA sequencing. Cellular and molecular tools for use in crayfish are underdeveloped compared to other model organisms, and cultured embryonic cells could provide a new testbed for those techniques.
Keywords: None provided.
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