Coutinho Carneiro and colleagues 2025

Coutinho Carneiro V, Schäfer I, Diaz-Larrosa JJ, Böhl E, Hahn T, Hempelmann J, Venkatesh G, Nagarajan S, Roy S, Ng YL, Böhl F, Zibek S, Lyko F. 2025. Establishment and characterization of biofloc culture for marbled crayfish. Frontiers in Aquaculture 4. https://doi.org/10.3389/faquc.2025.1580560

Abstract

Biofloc technology is an innovative aquaculture approach that enhances water quality and reduces waste through the development of beneficial microbial communities. Marbled crayfish (Procambarus virginalis) are a newly discovered freshwater crayfish species that are distinguished by asexual reproduction, a genetically homogeneous all-female population structure and considerable robustness. These traits are attractive features for closed-system aquaculture production, which also mitigates the invasive risk associated with the species. Here we describe the establishment and characterization of biofloc culture for marbled crayfish. Juvenile crayfish raised in biofloc systems displayed robust growth with a six-fold weight increase over three months, comparable with other crustaceans under similar conditions. Metagenomic analysis revealed that biofloc communities consisted mostly of beneficial Gammaproteobacteria and Bacillariophyceae, with variations by environmental factors, such as temperature and light exposure. DNA methylation profiling identified systematic changes in response to biofloc environments, highlighting the potential of DNA methylation analysis for environmental biomarker identification. Finally, nutritional analysis showed that biofloc-grown crayfish produced meat with a highly favorable protein-to-fat ratio, while their shells retained considerable amounts of chitin, an important raw material for bioplastics production. Taken together, our study is the first to establish marbled crayfish biofloc culture and to characterize the interaction between the animals and the production system.

Keywords: biofloc technology • sustainability • aquaculture • marbled crayfish • epigenetics •
chitin

 

Aluma and colleagues 2025

Aluma MO, Kaldre K, Strand DA, Hurt M, Pukk L. 2025. eDNA-based detection of invasive crayfish and crayfish plague in Estonia. Environmental DNA 7(3): e70107. https://doi.org/10.1002/edn3.70107

 

Abstract

 

In Estonia, three invasive North American crayfish species—Pacifastacus leniusculus, Faxonius limosus, and Procambarus virginalis—have been detected through the annual monitoring program. To protect Astacus astacus, the only native freshwater crayfish species in Estonia, rapid and effective conservation-based management actions are necessary. Recently, the environmental DNA (eDNA) approach has been increasingly used in Europe to detect crayfish species and the crayfish plague pathogen Aphanomyces astaci. Our study explored the potential of integrating the eDNA approach into ongoing annual monitoring programs for invasive crayfish species and A. astaci. We also evaluated the relationship between eDNA concentration and signal crayfish population density at a single location. We filtered 139 eDNA samples from 16 water bodies and screened them for A. astacus, P. leniusculus, and A. astaci using singleplex qPCR assays. A subset of the samples was also screened for P. virginalis and F. limosus. Crayfish eDNA was detected in nine out of 14 water bodies where presence was confirmed by trapping, resulting in a 64% detection efficiency. Detection of P. virginalis was only observed in samples with amplifications below the limit of detection, and A. astaci eDNA was found in only one water body hosting invasive crayfish species. Although we could not establish a convincing quantitative correlation between the estimated P. leniusculus eDNA concentration and crayfish population density, we conclude that the eDNA approach is promising and, with further optimization, it can be integrated into routine monitoring of crayfish and crayfish plague pathogen as a supplement to traditional trapping methods.

 

Keywords: None provided. 

Short course on Marmorkrebs

The Invasive Species Center is offering a short course on Marmorkrebs.

The course description:

This short course will provide an overview on crayfishes, with a particular focus on marbled crayfish biology, spread, and impacts.

Lesson 1: Introduction to Aquatic Invasive Species

Lesson 2: Introduction to Crayfishes

Lesson 3: Marbled Crayfish Biology

Lesson 4: Marbled Crayfish Impacts

Lesson 5: Preventing the Spread of Invasive Crayfishes

This course takes about 2 hours. Score an 80% on the final exam, and score a certificate of completion! 

The Center’s Instagram page has a discount code that lets you take the course for free until the end of May! If you happen to be reading this in June 2025 or later, sorry that it is no longer free, but should still be affordable.

External links

Invasive Species Training

Invasive Species Center on Instagram (contains discount code)